The D500 has AF adjustment which can work along with this product.

The Datacolor SpyderLensCal Autofocus Calibration Aid is designed for use with DSLR's and may not be good for all the needs of cinema video lens calibration.

Yes, both your setups can be used with this device as long each camera has micro focus adjust.

No additional software is required.

Testing and fine tuning AF accuracy is a way to insure tac sharp images, especially important with todays high resolution optics and sensors. This feature is found in the more advanced and pro model digital cameras. Canon calls theirs AF Microadjustment and on the 5DmkII it can be accessed in Custom Function C.Fn III-8. Mentioned on page 200 of the instruction manual. The 5D mkIII is not a custom function but is under the AF menu.Both the SpyderLensCal Autofocus Calibration Aid from Datacolor & MkII Focus Calibration System from LensAlign use a detailed focusing target plus a ruler to see correct fall off. The LensAlign includes True Parallel Alignment which is the basis that provides visual verification of perfect camera positioning during AF calibration. The orientation of your camera is essential for accurate analysis and adjustment of the camera's auto-focus systems.For more information on Canons AF Microadjustment: http://bhpho.to/1g0Ighg

Yes the Datacolor SpyderLensCal Autofocus Calibration Aid is also compatible with the D800.

Yes it will , micro adjustment

Some zoom lenses and some cameras can be calibrated just fine. However, if you have a lens which requires different calibration settings at different focal lengths, and you camera only accommodates one calibration setting, then you will need to send the lens to the mfg to be calibrated before you can do the autofocus micro adjustment calibration in-camera. For instance, my old Tamron 70-200mm lens had been dropped a few times. On most of my bodies, at 70mm, I needed to adjust the MF adjustment to -5, at 135mm it needed 0 calibration, and at 200mm it needed to be +10. My old canon 7D only has one MF adjustment setting per lens. I sent the lens to Tamron, and when it came back I was able to adjust it perfectly on the 7D. However, that same lens (before sending to Tamron) could be calibrated on my 5D Mark IV. By setting the 70mm setting to -5 and the 200mm setting to +10, the calibration settings worked throughout the entire focal range.